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101.
Vittori Antisari Livia Bianchini Gianluca Cremonini Stefano Di Giuseppe Dario Falsone Gloria Marchesini Marco Marvelli Silvia Vianello Gilmo 《Journal of Soils and Sediments》2016,16(2):645-662
Journal of Soils and Sediments - This study investigated a Lateglacial to Holocene sedimentary sequence derived from a small catchment located at San Lazzaro di Savena in the surroundings of... 相似文献
102.
103.
Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study 下载免费PDF全文
Angela Boggero Alberto Basset Martina Austoni Enrico Barbone Luca Bartolozzi Isabella Bertani Alessandro Campanaro Antonella Cattaneo Fabio Cianferoni Giuseppe Corriero Ambrosius Martin Dörr A. Concetta Elia Gentile Francesco Ficetola Lyudmila Kamburska Gianandrea La Porta Sara Lauceri Alessandro Ludovisi Elda Gaino Enzo Goretti Massimo Lorenzoni Marina Manca Aldo Marchetto Giuseppe Morabito Francesco Nonnis Marzano Alessandro Oggioni Cataldo Pierri Nicoletta Riccardi Giampaolo Rossetti Nicola Ungaro Pietro Volta Silvia Zaupa Diego Fontaneto 《水产资源保护:海洋与淡水生态系统》2014,24(6):841-852
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104.
Mariam Hamdan Ana Tomás‐Vidal Silvia Martínez Jesus Cerezo‐Valverde Francisco J Moyano 《Aquaculture Research》2014,45(12):2048-2056
An in vitro model designed to assess protein bioavailability in diets for growing Octopus was developed. The model required a previous assessment of some functional features of protein digestion in this species like the main producing organs, optimum pH for activity and total production per g tissue. The main producing organs identified were the salivary glands and the hepatopancreas (HP), being optimum pH for protease activity quite different in both organs (mostly alkaline in the posterior salivary glands and acid in the HP). In spite of the high‐specific protease activity measured in the salivary glands, a major role of the HP in protein hydrolysis is suggested due to the much bigger size of this viscera. All this information was used as a basis to develop an in vitro two‐step hydrolysis process, which simulated protein hydrolysis performed by these two organs using the Octopus enzymes. The assay was used to evaluate differences in amino acid bioavailability from several protein sources (casein, gelatin, fish meal, squid meal and krill meal) that could be used as feed ingredients for this species. As significant differences were detected both in total amount and in rate of release of the amino acids from such proteins, the model is proposed as a complementary tool in the selection and nutritional evaluation of protein ingredients to be used in diets for Octopus. 相似文献
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106.
Dermatophytosis is a common zoonotic disease, and one of its clinical presentations in the dog is nodular dermatophytosis (kerion). Because the infection is located within the dermis, routine diagnostic tests such as a Wood's lamp examination, microscopic examination of hair shafts for fungal elements and fungal culture can yield negative results. In such cases, histopathological examination with routine and special stains (periodic acid-Schiff, Gomori methenamine silver) is required to confirm the diagnosis. Nodular dermatophytosis in 23 dogs of different breed, age and sex with single or multiple nodules is described. Twelve dogs had a single nodule, and 11 dogs showed multiple lesions. Wood's lamp examination was negative in all cases. Microscopic examination of plucked hairs showed arthrospores in 8 of 23 cases. Skin scrapings in mineral oil looking for arthrospores and/or hyphae were positive in 12 cases. Impression smears of exudates were diagnostic in 21 of 23 cases (91%), showing arthrospores within fragments of hair shafts or free among neutrophils and macrophages (pyogranulomatous inflammation). Histopathology was performed in two cases. Fungal culture was positive for Microsporum canis in 16 dogs and for Microsporum gypseum in one dog. In six cases, the causative agent was not identified by fungal culture. All dogs were treated with systemic antifungal therapy and in eight cases with concurrent antibiotic therapy. Nodular dermatophytosis resolved in all dogs with the prescribed treatments within 4 to 8 weeks. Transmission to people or other pets in the home was not found. 相似文献
107.
Valentina Mariani Simona Palermo Silvia Fiorentini Alessandra Lanubile Elisabetta Giuffra 《Veterinary immunology and immunopathology》2009,131(3-4):278-284
The intestinal epithelial cells (IEC) play an important role in the immune system of swine, protecting against infectious and non-infectious environmental insults. The IEC participate in the innate immune response of the intestine through different mechanisms such as barrier function, mucus secretion, antibacterial peptide synthesis and participation in the cytokine/chemokine networks.Most of the current knowledge of intestinal cell functions has come from studies conducted on cell cultures generated from human cancers or from classical animal models. However, because the molecular and cellular elements of the immune system have been selected over evolutionary time in response to the species-specific environment, models of immune function based on mouse and human need to be applied cautiously in pig. Few models of swine small intestine epithelium exist and these are poorly characterised. In the present study we characterised the basal expression of epithelial and immune-related genes of two pig small intestine cell lines, IPEC-J2 and IPI-2I, under different culture conditions. These data represent essential background information for future studies on pig-intestinal pathogen interactions. 相似文献
108.
Silvia Pietschmann Katrin Hoffmann Michael Voget Ulrich Pison 《Veterinary research communications》2009,33(6):489-505
The therapeutic value of antibiotics depends on the susceptibility of the infecting microorganism and the pharmacological
profile of the drugs. To assess the value of an antibiotic combination of polymyxin B and miconazole this study examined the
in vitro synergistic potential of the two drugs on Gram-negative and Gram-positive bacteria and yeast. Antifungal and antibacterial
activity was tested by minimum inhibitory concentration (MIC) of broth macrodilution and urea broth microdilution, by fluorescence
microscopy and flow cytometry. Synergism was calculated using the fractional inhibitory concentration index (FICi). With Staphylococcus intermedius as target we found up to an eightfold reduction of the individual MICs when both drugs were combined. However, the FICi was
0.63 suggesting no real interaction between the two drugs. With Escherichia coli, Pseudomonas aeruginosa, and Malassezia pachydermatis as targets the antimicrobial drug combination reduced the MICs of polymyxin B and miconazole from fourfold to hundredfold
resulting in FICi between 0.06 and 0.5 which defines a synergistic action. Thus, if polymyxin B and miconazole are combined
their effect is greater than the sum of the effects observed with polymyxin B and miconazole independently, revealing bactericidal
and fungicidal synergism. Our results indicate a strong therapeutic value for the combination of these antimicrobial agents
against Gram-negative bacteria and yeast and a weaker value against Gram positive bacteria for clinical situations where these
pathogens are involved. 相似文献
109.
Soil compaction affects physical soil condition, in particular aeration, soil strength, and water availability and has adverse effects on plant growth. Bulk density is the most frequently used indicator to describe the state of compaction of a soil. However, this parameter lacks a direct functional relationship with plant growth. Various indicators have been proposed to simultaneously characterize the state of compaction of agricultural soil and its suitability for plant growth. This paper examines and compares the critical limits for crop plant growth based on three of these indicators: packing density, least limiting water range, and S parameter (the latter is the slope of the soil water‐retention curve in the inflexion point). In a first step, we reviewed the literature for published optimum and limiting values of bulk density and found that these values were highly dependent on clay and silt content. Converting them into corresponding values of packing density (composite index of bulk density and clay content), a value of 1.70 was found to effectively distinguish between optimum and limiting soil conditions for plant growth. In a second step, the packing density of 59 soil horizons sampled in N Switzerland was compared with the least limiting water range and the S parameter of these soil horizons (both determined by means of pedotransfer functions taken from the literature). A linear relationship between the three parameters was found, which allowed for a comparison of the published critical limits for plant growth based on these parameters. The critical limits of the three indicators, which had been postulated independently of each other in the literature, were found to agree well with each other. This means that all of them could equally be used to describe the compaction state of a soil and its physical suitability for plant growth. However, the proposed critical limits of packing density, least limiting water range, and S parameter still need further validation by field studies relating plant growth to soil compaction. 相似文献
110.
Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer 总被引:3,自引:0,他引:3
Furlanello T Tasca S Caldin M Carli E Patron C Tranquillo M Lubas G Solano-Gallego L 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):42-46
BACKGROUND: Artifactual changes in blood may occur as a consequence of delayed analysis and may complicate interpretation of CBC data. OBJECTIVE: The aim of this study was to characterize artifactual changes in canine blood, due to storage, using the ADVIA 120 hematology analyzer. METHODS: Blood samples were collected into EDTA from 5 clinically healthy dogs. Within 1 hour after blood sample collection and at 12, 24, 36 and 48 hours after storage of the samples at either 4 degrees C or room temperature (approximately 24 degrees C), a CBC was done using the ADVIA 120 and multispecies software. A linear mixed model was used to statistically evaluate significant differences in values over time, compared with initial values. RESULTS: The HCT and MCV were increased significantly after 12 hours of collection at both 4 degrees C and 24 degrees C, and continued to increase through 48 hours. The MCHC initially decreased significantly at 12-24 hours and then continued to decrease through 48 hours at both temperatures. Changes in HCT, MCV, and MCHC were greater at 24 degrees C than at 4 degrees C at all time points. A significant increase in MPV and a decrease in mean platelet component concentration were observed at all time points at 24 degrees C. Samples stored at 24 degrees C for 48 hours had significantly higher percentages of normocytic-hypochromic RBCs, and macrocytic-normochromic RBCs, and lower platelet and total WBC counts. CONCLUSIONS: Delayed analysis of canine blood samples produces artifactual changes in CBC results, mainly in RBC morphology and platelet parameters, that are readily detected using the ADVIA 120. Refrigeration of specimens, even after 24 hours of storage at room temperature, is recommended to improve the accuracy of CBC results for canine blood samples. 相似文献